Expression, purification and characterization of the ubiquitous protein kinase C-related kinase 1
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چکیده
منابع مشابه
Expression, Purification, and Characterization of Protein Kinase C-r*
Of the recently described members of the protein kinase C (PKC) family (-6, -6, -c), no detailed properties of the purified enzymes have been presented. Here we describe the expression of PKC-c in insect cells using a baculovirus vector. The recombinant enzyme has been purified to homogeneity by sequential chromatography on DEAE-cellulose, serine-Sepharose, Mono Q, and Superose 12; the protein ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1995
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3090315